Crystals of 70S ribosomes from thermophilic bacteria are suitable for X-ray analysis at low resolution
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چکیده
Single crystals of 70S ribosomes from Thermus thermophdus and from the wild-type and a mutant of Bacdlus stearothermophdm have been obtained. Using synchrotron X-ray beam, these crystals diffract to 19 ,~ and 35 A, respectively, and can be ~rradlated at temperatures of 110-120 K for days without noticeable decay Diffraction data sets to 22 ,~ and to 33 A with reasonable R scale v, ere collected from the crystals of 70S from T thermophtlus The cell dimensions (a = b = 524 A, ~ = 315 ,~), the symmetry (P41212 or P43212) and the density (1 063 g/cm 3) of these crystals indicate that there is one 70S ribosome per asymmetnc unit
منابع مشابه
Crystals of complexes mimicking protein biosynthesis are suitable for crystallographic studies.
A complex of 70S ribosomes from Thermus thermophilus together with an average of 1.5-1.8 equivalents of PhetRNA(Phe) and a short mRNA chain, composed of 35 +/- 5 uridines, was crystallized under the conditions used for the growth of crystals of isolated ribosomes from the same source. Considering the reproducibility of their growth, their internal order and their shape, the crystals of the comp...
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تاریخ انتشار 2002